Determination of Homogentisic Acid in Bamboo Shoots by Column Chromatography on Sephadex G-10 and Reversed-Phase High-Performance Liquid Chromatography

Separation of Somatropin and Its Degradation Products by High-Performance Liquid Chromatography Using a Reversed-Phase Polymeric Column

The accurate prediction of protein stability is one of the most challenging goals in protein formulation and delivery. In this study, a gradient RP-HPLC method is described for the separation of human growth hormone (hGH) variants as deamidated and oxidized forms. The methodology employed a polymeric poly (styrene-co-divinylbenzene) column and a 1mL/min flow rate of a linear gradient of 0.1% v/...

Separation of Somatropin and Its Degradation Products by High-Performance Liquid Chromatography Using a Reversed-Phase Polymeric Column

The accurate prediction of protein stability is one of the most challenging goals in protein formulation and delivery. In this study, a gradient RP-HPLC method is described for the separation of human growth hormone (hGH) variants as deamidated and oxidized forms. The methodology employed a polymeric poly (styrene-co-divinylbenzene) column and a 1mL/min flow rate of a linear gradient of 0.1% v/...

Reversed-phase high-performance liquid chromatography.

Determination of ethylenediaminetetraacetic acid in foods by reversed-phase high-performance liquid chromatography.

A convenient HPLC method for the quantitative determination of EDTA in foods was developed. EDTA in food samples was easily extracted with water by ultrasonication. After converting to Fe(III) complex in the presence of Fe(III) ions, EDTA was separated on a reversed-phase C30 column and detected with ultraviolet detection (260 nm). Citrate and malate, which are present in many foods, also forme...

determination of gamma - aminobutyric acid by high-performance liquid chromatography

gamma-aminobutyric acid is the main inhibitory amino acid neurotransmitter in several areas of the brain. measurement of gamma-aminobutyric acid in the brain will help elucidate its metabolic rote and diagnostic value. the liquid chromatography (lc) system consisted of a delivery pump. male rats (150-250g) were used in this study. the brain was rapidly removed from decapitated animals. trie chr...